Fully modified 2' MOE oligonucleotides redirect polyadenylation
نویسندگان
چکیده
منابع مشابه
Generation of long, fully modified, and serum-resistant oligonucleotides by rolling circle amplification.
Rolling Circle Amplification (RCA) is an isothermal enzymatic method generating single-stranded DNA products consisting of concatemers containing multiple copies of the reverse complement of the circular template precursor. Little is known on the compatibility of modified nucleoside triphosphates (dN*TPs) with RCA, which would enable the synthesis of long, fully modified ssDNA sequences. Here, ...
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The first part of this work deals with the thermal stability of oligonucleotides modified with acetylaminofluorene and aminofluorene, respectively. The complementary oligonucleotides d(CGCG), d(CGTACG) and d(AATTGCAATT) have been studied by ultraviolet absorption and circular dichroism. In high salt concentration and at low temperature, the three oligonucleotides form double-stranded helices wh...
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2'-O-Methyl oligoribonucleotides with four kinds of 2'-O-modified uridine derivatives were synthesised. Their duplex stability, hydration behavior and exonuclease resistance were studied by spectroscopic analyses and molecular dynamics simulations. Consequently, 2'-O-modification of the uridine residue with 2-carbamoylethyl or 2-(N-methylcarbamoyl)ethyl groups resulted in a significant improvem...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2001
ISSN: 1362-4962
DOI: 10.1093/nar/29.6.1293